Coding

Part:BBa_K4825038:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


OPT-Cry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 268
    Illegal BglII site found at 405
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Test.


Source

Saccharomyces cerevisiae

References

1. Aza P, Molpeceres G, de Salas F, Camarero S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci. 2021 Apr;78(7):3691-3707. doi: 10.1007/s00018-021-03793-y. Epub 2021 Mar 9. PMID: 33687500; PMCID: PMC8038962. 2. 孙明, 郭素霞, 王鹏霞, 彭东海, 阮丽芳, 喻子牛. Nematicidal bacillus thuringiensis crystal protein gene cry1518-35. WO2010078708A1 [P]. 2010-07-15.